| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1062-1 | ||||
Resumo:Bees have economic and environmental value for society, being important pollinators and producers of honey, a food and drug with antimicrobial activity used by humanity for centuries. The pharmacological properties of honey are a result of its chemical composition, often determined by fermentative processes mediated by microorganisms. With increasing agricultural expansion, it has become increasingly common for bees to come into contact with xenobiotics. Thus, the present work aimed to analyze the resistance profile of bacteria isolated from honey and propolis of the Jataí bee, in addition to evaluating the antimicrobial properties of the isolates. Honey and propolis from the Jataí bee were collected in boxes located in the Integral Protection Conservation Unit of Buraco do Padre, located in the region of Ponta Grossa – PR. The collected samples were diluted in sterile saline solution (NaCl 0.95%), sown in Petri dishes containing Luria Bertani agar (LB) and incubated in an oven at 30 °C for 72 hours. After growth, it was possible to isolate 13 strains present in honey and propolis. For the analysis of the resistance profile, the isolated strains were grown in LB broth, plus the herbicides Roundoup, Aminol, Atectra and Heat in field dose and 10x field dose. To analyze the resistance profile of the bacteria against antibiotics, the bacterial strains were inoculated in Petri dishes containing LB agar culture medium containing the antibiotics tetracycline, ciprofloxacin, doxycycline and erythromycin at resistance doses (according to BrCAST, with cutoff stations for Enterobacteriaceae) and at doses with a concentration of 10x that of cut and incubated in an oven at 30 °C for 72 hours. The antimicrobial analysis of honey against ATCC bacteria was performed using the technique of direct contact between the isolated strains of honey and propolis and strains of Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Proteus mirabilis ATCC 252933 and Enterococcus faecalis ATCC 19422, where the isolated strains were inoculated in Petri dishes on the culture medium in direct contact with the ATCC strains and incubated in an oven at 30 °C for 72 h. The reading was performed qualitatively by the formation of inhibition halos. When analyzing the growth profile of the isolates against the herbicides tested, it can be observed that the strains present a profile of sensitivity to the herbicides Randoup and Aminol, which is an indication of the absence of selection pressure of these herbicides in the nest analyzed. However, the isolates showed resistance to the antibiotics tetracycline, ciprofloxacin and erythromycin, possibly due to the antimicrobial properties of honey and propolis itself. Additionally, it was possible to verify the inhibition capacity of Staphylococcus aureus bacteria when in contact with five isolates but not for the gram-negative bacteria tested (E. coli, P. mirabilis). The resistance profile observed for bacterial isolates is selected by selection pressure directed by the antimicrobial properties of honey and propolis. It is also possible to infer the absence of resistance of the isolates against herbicides harmful to the behavior and survival of the bees. Palavras-chave: Antimicrobial activity, ATCC bacteria, bees, herbicides | |||||